High performance liquid chromatography is at present one of the classiest tool of the analysis. The estimation of raltegravir and lamivudine was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of acetonitrile: phosphate buffer mixed in the ratio of 45:55 % v/ v. Inertsil ODS 3V C18 column (4.6 x 150 mm, 5 m) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out by using PDA detector at 275 nm. The solutions were chromatographed at a constant flow rate of 1.0 ml/min. the linearity range of raltegravir and lamivudine were found to be from 150-450 g/ml of raltegravir and 50-150 g/ml of lamivudine. Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 100.36 and 100.30% of raltegravir and lamivudine. LOD and LOQ were found to be within limit.
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